Influence of ulinastatin on renal pathology and relevant indexes of urine in rats with acute kidney injury caused by sepsis

Objective To discuss the influence of ulinastatin on renal pathology and relevant indexes of urine in rats with sepsis-induced acute kidney injury(AKI) caused by cecal ligation and puncture(CLP).Methods 55 cases of healthy and male SD rats were randomly divided into 3 groups: control group(n=5),model group(n=25),and ulinastatin group(n=25).The last two groups were then randomly divided into 5 points in time including 1 h,6 h,12 h,24 h,and 48 h with 5 rats in each time point.The sepsis model was duplicated by CLP.After successfully establishing the model,in the ulinastatin group,100 000 U/kg ulinastatin was administered for intravenous injection.The blood and urine was collected in the set 5 points in time.These rats were executed and relevant indexes like serum creatinine(SCr),blood urea nitrogen(BUN),kidney injury molecule-1(KIM-1),interleukin-18(IL-18),and neutrophil gelatinase associated lipocalin(NGAL) was tested and analyzed.Changes of renal pathology were observed under light microscope.Results In the model group,concentrations of SCr and BUN 6 h after CLP started to rise,reached peak in 24 h,and decreased in 48 h,which were all higher than those in the control group(P0.05).After ulinastatin therapy,the concentrations of Scr and BUN in 12 h,24 h,and 48 h were all lower than those in the corresponding points in time in the model group(P0.05).Contents of KIM-1,NGAL,and IL-18 1 h after CLP started to increase,reached peak in 12 h,and decreased in 24 h in the model group were all remarkably higher than those in the control group.After giving ulinastatin,the contents of KIM-1,NGAL, and IL-18 in 6 h,12 h,24 h,and 48 h were greatly lower than those in the corresponding points in time in the model group(P0.05).In the ulinastatin group,changes of renal pathology was better than that of the model group.Conclusion Ulinastatin plays the role of renal protection in AKI caused by sepsis.