Protective effects of ulinastatin on acute kidney injury in rats with sepsis

Objective To investigate the effects of ulinastatin on acute kidney injury in rats with sepsis. Methods Fifty-five healthy male sprague-dawley rats were randomly divided into 3 groups: normal control, model group, ulinastatin group.The rat models of sepsis were established by caecal ligation and puncture(CLP).In treatment groups, rats were treated with ulinastatin through caudalis injection after CLP surgery.Each group was divided into 1, 6, 12, 24, 48 hour subgroups with 5 rats. Rats were sacrificed and tissue samples including kidney and blood samples were collected.The kidney function,urine level of kidney injury molecule-1(KIM-1), neutrophil gelatinase lipocalin(NGAL) and interleukin-18(IL-18) were measured.Results Compared with CLP model group, renal function, the levels of KIM-1, NGAL and IL-18 in urine, significantly decresed in Ulinasta group(P 0.05). Conclusion Ulinasta can improving renal function duringAKI in the rat model of sepsis.