Determination of imperatorin and isoimperatorin in yuanhu zhitong jiaonang by UPLC

To establish an UPLC method for detecting content of imperatorin and isoimperatorin in Yuanhu Zhitong Jiaonang. An ACQUITY UPLC BEH C l8( 1. 7μm,2. 1 × 50 mm) was used,and the mobile phase was methanol and water by gradient elution mode,column temperature was 30 ℃,at a flow rate of 0. 3 ml / min and detection wavelength of 248 nm. The results showed that the linear range of imperatorin was 1. 305 ～13. 05μg / mL,the regression equation was as follows: Y = 13828X + 2962( r = 0. 9999); The linear range of isoimperatorin was 0. 596 ～ 5. 96μg / mL,the regression equation was as follows: Y = 10634X + 1128( r =0. 9999); The average recoveries were 98. 54%( RSD = 0. 76%) and 98. 88%( RSD = 0. 87%) respectively,the limit of detection of imperatorin and the limit of quantification of imperatorin were 0. 012 ng and 0. 04 ng,the limit of detection and the limit of quantification for isoimperatorin were 0. 27 ng and 0. 92 ng.