Effect of Mongolian medicine Garidi-13 pill on IL-1β,TNF-αprotein and nerve cell apoptosis of cerebral ischemia hippocampus tissue of rats

Objective To investigate the effects of Mongolian medicine Garidi-13 pill on inflammatory cytokines interleukin-1β( IL-β) and tumor necrosis factor-α( TNF-α) expression and nerve cell apoptosis of cerebral ischemia hippocampus tissue of rats. Methods Two times of blood injection to the cistema magna was used to make the rat model of cerebral ischemia. Brain tissue was acquired at each time point. Flow cytometry and ELISA were used to detect the number of apoptotic cells,IL-1β and TNF-α content in hippocampus tissue. Results Compared with normal group,IL-1βand TNF-αin hippocampus were increased,apoptosis percentage number was increased of model group( P0. 05). Conclusions Garidi-13 can reduce the number of apoptosis nerve cell in ischemia brain tissue and reduce the severity of ischemic injury,its mechanism may be related to reduce IL-1β and TNF-αcontent of brain.