VEGF mRNA Expression in Breast Cancer and Its Relation to Clinicopathological Parameters

Objective To investigate the expression of VEGF in breast cancer tissue and its relation to clinicopathological and other molecular parameters.Methods The expression of VEGF mRNA in 48 cases of breast carcinoma and 20 cases of breast fibroadenoma issues were detected by fqRT-PCR and the protein expression of VEGF in those issues were detected by SABC immunohistochemistry.The protein expression of HER-2,ER,PR in 48 cases of breast carcinoma were detected by using SABC immunohistochemistry.The relationship of its expression with VEGF,HER-2,ER,PR were analyzed.Results By RT-PCR method,the 2-ΔCt values of VEGF121 mRNA and VEGF165 mRNA of the breast carcinoma and breast fibroadenoma tissues were 0.5733±0.1764,0.310±0.098 and 0.2961±0.084,0.2657±0.0342 respectively.The positive expression rates of VEGF mRNA were significantly higher in breast carcinoma than those in breast fibroadenoma tissues,there were significant difference among the two groups(P0.05).The positive expression rates of VEGF in breast carcinoma had no significant correlation with the age,lymph node metastasis,tumor size,differentiation degree,pTNM stages of the patient(P0.05).The expression of HER-2 were 18.8% and 0% in breast carcinoma and fibroadenoma issues respectively.there were significant difference among the two groups(P0.05).The expression of HER-2 were negatively associated with that of ER and PR(γ=-0.335,P=0.005;γ=-0.300,P=0.012).The expression of VEGF had no correlation with that of HER-2,ER,PR(P0.05) by Spearman analysis.Conclusion Overexpression VEGF mRNA is over-expressed and not correlated with the ER,PR status.HER-2 may serve as an important prognostic factor of breast cancer