Construction and identification of lentiviral vector of RNA interference of CRBP-1 gene

Objective:To construct a lentiviral vector of RNA interference(RNAi) of rat cellular retinol-binding protein I(CRBP-I) gene.Methods:The effective sequence of siRNA targeting CRBP-I gene was confirmed in our previous study.The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed,synthesized and cloned into the pGCL-GFP vector,to construct a lentiviral vector which expressed short hairpin RNA(shRNA),and it was identified by PCR and DNA sequencing.Results:PCR identification and DNA sequencing demonstrated that insertion of oligonucleotide of the lentivirus RNAi vector containing CRBP-I shRNA was right.Conclusion:The lentivirus RNAi vector of rat CRBP-I was constructed successfully.