Construction and Sequencing of lentiviral Vector of Short Hairpin RNA of Rat CRBP-1 Gene

【Objective】To construct and sequence a lentiviral vector of short hairpin RNA(shRNA)of rat cellular retinol-binding protein I(CRBP-I) gene.【Methods】The effective sequence of RNAi targeting CRBP-I gene was confirmed in our previous study.The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed,synthesized and cloned into the pGCL-GFP vector to construct a lentiviral vector,which could express shRNA.The resulting lentiviral vector containing CRBP-1 shRNA was named LV-shCRBP-1,which was identified by PCR and DNA sequencing.【Results】PCR identification and DNA sequencing demonstrated that insertion of oligonucleotide of LV-shCRBP-1 was right.【Conclusion】The lentivirus RNAi vector of rat CRBP-I has been constructed successfully.