2009•Unpublished venueRequires access
Construction and identification of lentiviral vector of RNA interference of CXCR4 gene
Xingpeng Wang
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Abstract
Objective:To construct a lentiviral vector of RNA interference(RNAi)of human CXCR4 gene.Methods:The effective sequence of siRNA targeting CXCR4 gene was confirmed in our previous study.The complementary DNA containing both sense and antisense Oligo DNA of the targeting sequence was designed,synthesized and cloned into the pGCL-GFP vector,to construct a lentiviral vector which expressed short hairpin RNA(shRNA),and it was identified by PCR and DNA sequencing.Results:PCR identification and DNA sequencing demonstrated that insertion of oligonucleotide of the lentivirus RNAi vector containing human CXCR4 shRNA was right.Conclusion:The lentivirus RNAi vector of human CXCR4 was constructed successfully.