Ploidy Identification and Chromosome Doubling in Microspore Regenerated Plantlet of Kale

In order to increase the proportion of diploid kale microspore plantlets,the ploidy level of microspore plantlets( Bolangyehongxin,Zhouyehongxin,Hongou,Baiou) was identified by the DNA flow cytometry,and the haploid plantlets and isolated microspore were treated with colchicines solution. The results showed that the haploid plantlets were cultured in MS medium containing 75 mg / L colchicines,the diploid induction rate of Bolangyehongxin and Zhouyehongxin were 20. 0% and 5. 0% respectively,the effect was worse; the root of haploid plantlets were soaked in 1 000 mg / L colchicine,the diploid induction rate of the above two varieties were 53. 3% and 25. 0% respectively,the effect was better; the isolated microspore was treated with 50 mg / L colchicine for 36 h,the diploid induction rates of the four varieties were the highest,the Hongou double haploid ratio was the highest with 55. 6%.