Effects of triptolide on expression of inducible nitric oxide synthase and production of nitrogen monoxide in microglia cells induced by β-amyloid

Objective To investigate the effects of triptolide(T10) on expression of inducible nitric oxide synthase(iNOS) and production of nitrogen monoxidum(NO) in microglia cells induced by β-amyloid(Aβ) 1-42.Methods The microglia cells were pretreated with 10-11-10-8 mol / L T10 for 12 h before 10 μmol / L Aβ1-42 were added,and incubated for 12 h together.The production of NO was determined by Griess reaction,and the expression of iNOS was determined by Western blotting.Results Expression of iNOS and production of NO in Aβ group were significantly higher than those in control group(P 0.01).T10 treatment reduced expression of iNOS and production of NO obviously induced by Aβ from microglia cells(P 0.05).Conclusion T10 significantly inhibited expression of iNOS and production of NO in microglia cells induced by Aβ.T10 might provide a novel therapy for AD.