2005•Unpublished venueRequires access

The study of the production of nitric oxide in β-Amyloid1-42 stimulated microglia in vitro

Nie Yong-hui, Wang Luning, Ling Ye

Abstract

Objective:To explore the production of nitric oxide(NO) in β-Amyloid1-42 stimulated microglia in vitro.(Methods:We) cultured murine microglia BV-2 cells as the model of microglia in vitro.The production of nitric oxide(NO) and the activity of inducible nitric oxide synthase(iNOS) were studied in Aβ 1-42 stimulated BV-2 microglia.iNOS protein expression was examined by flow cytometry.Results:NO production was induced by Aβ1-42 in a dose and time dependent manner,Aβ1-42 can also increase iNOS activity and iNOS protein expression in a dose and time dependent manner.iNOS maybe the main source of NO production.Conclusions:On exposure to Aβ microglia can secrete NO,which may trigger pathways of neuronal degeneration in Alzheimer's disease.amyloid beta-protein;microglia;nitric oxide

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Key concepts: Microglia, Nitric oxide, Nitric oxide synthase, In vitro, Chemistry, Cell biology, Flow cytometry, Immunology