Effect of Salinities Stress on Seed Germination of Glycine. Soja

Wild soybeans(Glycine soja),the ancestors of cultivated soybean(Glycine max),are important sources of major genes for resistance to pests,diseases and environmental stresses.The study of salt stress on the seed germination is invaluable for efficient utilization of saline-alkali soil resources and wild soybeans.Wild soybean,collected from Dezhou,Shandong province,were treated with NaCl,Na2SO4,Na2CO3 and the mixture of three solutions.Seed germination percentage,viability,index and the growth of radicle and hypocotyl of annual wild soybean were measured and analyzed.Results showed seed germination percentage,germination viability,germination index and the growth of radicle and hypocotyl annual wild soybean reduced with increasing of salt concentrations of Na2CO3 and the mixture.Low concentrations of Na2SO4(10-50 mmol·L-1),Na2CO3(0-10 mmol·L-1)promoted germination of seeds,while high concentrations of NaCl(200 mmol·L-1),Na2SO4(≥200 mmol·L-1)and Na2CO3(≥75 mmol·L-1)restrained it.Under low concentration of salt solution(10 mmol·L-1 Na2SO4),the radicel length of Glycine soja was longer than that of the control(distilled water).Results suggest that lower concentration of salt solution simulated the growth of radicle and hypocotyl of wild soybean,and the radicle is more sensitive to salt stress than that of hypocotyl.