Optimized compatibility of sedative-hypnotic components in Ziziphi Spinosae Semen by uniform design

Objective To optimize the compatibility of three sedative-hypnotic components(such as saponin,flavone,and alkaloid) in Ziziphi Spinosae Semen.Methods Seventy mice were divided into seven(control and experimental A—F) groups by uniform design,and their autonomic activites and synergistic effect were observed.After ip administration of pentobarbital sodium above threshold dose(45 mg/kg),taking sleep latency and sleep duration of mice as evaluating indicators,the best compatibility was screened.Then a further experiment for comparing and verifying the efficacy of the best compatibility was conducted.Results Compared with the control group,autonomic activities of mice in experimental group A,C,and E were reduced(P0.05,0.01),and time of standing in groups C and D was slightly reduced(P0.05).In the synergistic effect of pentobarbital sodium above threshold dose,sleep latency in experimental groups C—F was remarkably shortened(P0.01) and sleep duration in experimental groups B—F was prominently prolonged(P0.01).The results of multiple regression analysis showed that the optimized ratio of sedative and hypnotic active components in Ziziphi Spinosae Semen was saponin 200 mg/kg,flavone 0 mg/kg,and alkaloid 20 mg/kg.Conclusion It is feasible to screen the compatibility ratio by combining uniform design with pharmacodynamics.The verification experiment indicates that the sedative and hypnotic effect of the optimized compatibility components are the same as Ziziphi Spinosae Semen’s.