Experimental Study on Leizhi Capsule via Reducing Toxity and Increasing Effects of Tripterygium Glucosides in PAN Nephrosis Rats

Objective: Toxicity of tripterygium wilfordii often bring damage to digestive system,reproductive system,hematological and immune system,so reducing toxicity but maintaining therapy effect has been focused on tripterygium wilfordii studies in the past decade.Combining with traditional Chinese medicine compounds can reduce the toxicity but maintaining therapy effect of tripterygium.Whether Leizhi capsule(Ligustrum lucidum,Eclipta,plus tripterygium glucosides) can reduce toxity and increase effects of Tripterygium glucosides? So this study aims to explore the effects of Leizhi capsule in puromycin amino nucleoside(PAN) nephrosis rats.Method: Eighty male wistar rats were randomly divided into eight groups,including control group,model group,Leizhi capsule high-dosage and low-dosage group,tripterygium glucosides high-dosage and low-dosage group,triptolid group and valsartan group(with each group ten rats).PAN nephrosis medel was made by jugular vein injection of PAN(100 mg·kg-1),while control group rats were made by injection of physiological saline with equal volume.By irrigation stomach once a day for ten days,all rats had been given medicines as follows: physiological saline 2 mL for control group and model group,tripterygium glucosides 2 mg dissolved in Erzhi(containing Ligustrum lucidum and Drought Ephraim grass) solusion 1 mL or 1 mg dissolved in Erzhi solusion 0.5 mL for Leizhi capsule high-dosage or low-dose group,tripterygium glucosides 10,5 mg·kg-1 for Tripterygium glucosides high-dosage or low-dose group,triptolid 2 mg·kg-1 for triptolid group and valsartan 7.5 mg·kg-1 for valsartan group.The blood and urine samples were collected,liver and kidney tissues were processed after killed.The glomerular morphology and podocyte ultrastructure were observed respectively.Result: In day 5,model rats were in low spirits,with less urine,ascites,malnutrition and weight loss.From day 7 to day 10,the nephrotic syndromes were worst in model rats,but without skin edema.Some rats died of serious ascites,the mortality is 30%(3/10).Morphologic changes include epithelial cells degenerationrenal tubular and transparent cast,but there are no obvious changes in glomerulus and renal interstitial.Meanwhile,the podocyte processes effacement was obvious in model groups in electronic microscope.The nephrotic syndrome of PAN rats treated with Leizhi capsule and tripterygium glucosides significantly improved,compared with that in PAN model group and other treatment groups.The urinary protein excretion was decreased,plasma albumin was increased,and high cholesterol/triglyceride levels were remission,and anemia improved.Serum creatinine increased mildly(P0.05).In light microscope,epithelial cells degenerationrenal of renal tubular and transparent cast improved in both leizhi capsule and Tripterygium glucosides samples(P0.05).Meanwhile,the degree and area of podocte processes effacement was significantly reduced in both leizhi capsule and Tripterygium glucosides groups in electronic microscope(P0.05).Conclusion: Leizhi capsulecan reduce toxity and increase effects of Tripterygium glucosides by reducing proteinuria,improving anemia,lowering AST,improving podocte processes effacement,and reducing epithelial cells degeneration of renal tubular.