2009•Chinese Journal of Integrated Traditional and Western NephrologyRequires access
Influence of Tripterygium Wilfordii Glycoside Tablet on the Expression of Connective Tissue Growth Factor in Diabetic Nephropathy Rats
Liu Liqiu
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Abstract
Objective:To investigate the effect of tripterygium wilfordii glycoside tablets,on the expression of connective tissue growth factor(CTGF)in the diabetic kidney cortex and its relationship with tripterygium wilfordii glycoside tablets.Methods:Forty-eight Wistar rats were divided randomly into normal control group,diabetic nephropathic control group,tripterygium wilfordii glycoside tablets treated group.Rats in diabetic nephropathic control group and tripterygium wilfordii glycoside tablets treated group were given STZ(55 mg/kg)by intraperitoneal injection to establish animal model of diabetes.Four rats of each group were sacrificed at the end of the 4th、8th、12th week and to collect samples,including body weight、blood sugar、serum creatinine、blood urea nitrogen、cholesterol、triglyceride、24-hour urinary protein excretion.CTGF mRNA was measured by reverse transcriptase-polymerase chain reaction(RT-PCR).Results:(1)In diabetic nephropathic control group,the blood sugar、serum creatinine、blood urea nitrogen、cholesterol、triglyceride and 24-hour urinary protein excretion、the expression of CTGF mRNA in kidneys increased significantly(P0.01).(2)In diabetic nephropathic rats treated with tripterygium wilfordii glycoside tablets,the blood sugar、serum creatinine、blood urea nitrogen、cholesterol、triglyceride and 24-hour urinary protein excretion decreased significantly compared with diabetic nephropathic control group at the end of the 12th week,CTGF mRNA decreased significantly(P0.01).Conclusion:In diabetic nephropathy rats treated with tripterygium wilfordii glycoside tablets,24-hour urinary protein excretion、serum creatinine and blood urea nitrogen decreased significantly,compared with that in the diabetic nephropathy group,and CTGF mRNA expression of the kidney was significantly reduced.The result suggests tripterygium wilfordii glycoside tablets can effectively delay the progress of the diabetic nephropathy.