Cloning and Gentic Evolution of VP3 Gene of Goose Parvovirus Strain CHv

Primers were designed according to the genome of GPV B strain in Genbank.The DNA encoding VP3 structure protein of GPV CHv strain was amplified by PCR.The VP3 gene was cloned into pMD18-T vector and then sequenced.The DNA and amino acid sequence were compared with other GPV,MDPV,PPV,CPV and BPV.The results showed that the DNA of VP3 was 1 605 nt and coding 534 amid acids,shared 93.4%~99.8% nucleotide sequences identity with those published in Genbank,and shared 96.5%～99.3% amino acid sequence identity,which was the reasons that there was only one serum type of GPV.GPV CHv VP3 and MDPV shared 79.6% nucleotide sequences identity and 89.9% amino acid sequence identity,and shared less 30% nucleotide sequences identity with other parvovirus.