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Identification of linear B-cell epitopes on goose parvovirus non-structural protein and the gene sequence analysis

Junwei Wang

Open publisher page 1 citations

Abstract

In order to determining the B-cell epitopes on the non-structural protein(NS) of goose parvovirus(GPV),Seven DNA fragments were amplified by RT-PCR,and expressed in E.coli,which encoded 7 partially overlaying short peptides covering the C-terminal of NS protein(453aa to 627aa).The western blot analysis results show that the NS protein linear B-cell epitopes were located on 485aa to 514aa,498aa to 532aa,523aa to 556aa,543 to 573aa,564aa to 598aa and 599aa to 627aa.The deduced amino acid of epitope sequence was compared with those of 12 GPV and Muscovy duck parvovirus(MDPV) referenence strains in GenBank,and phylogenetic tree analysis showed that all the strains of GPV shared highly common identity compared with each other,but there were also some difference between different strains.Meanwhile,GPV and MDPV probably share the common epitopes.

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What this paper is about

In order to determining the B-cell epitopes on the non-structural protein(NS) of goose parvovirus(GPV),Seven DNA fragments were amplified by RT-PCR,and expressed in E.coli,which encoded 7 partially overlaying short peptides covering the C-terminal of NS protein(453aa to 627aa).The western blot analysis results show that the NS protein linear B-cell epitopes were located on 485aa to 514aa,498aa to 532aa,523aa to 556aa,543 to 573aa,564aa to 598aa and 599aa to 627aa.The deduced amino acid of epitope sequence was compared with those of 12 GPV and Muscovy duck parvovirus(MDPV) referenence strains in GenBank,and phylogenetic tree analysis showed that all the strains of GPV shared highly common identity compared with each other,but there were also some difference between different strains.Meanwhile,GPV and MDPV probably share the common epitopes.

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Available abstract

In order to determining the B-cell epitopes on the non-structural protein(NS) of goose parvovirus(GPV),Seven DNA fragments were amplified by RT-PCR,and expressed in E.coli,which encoded 7 partially overlaying short peptides covering the C-terminal of NS protein(453aa to 627aa).The western blot analysis results show that the NS protein linear B-cell epitopes were located on 485aa to 514aa,498aa to 532aa,523aa to 556aa,543 to 573aa,564aa to 598aa and 599aa to 627aa.The deduced amino acid of epitope sequence was compared with those of 12 GPV and Muscovy duck parvovirus(MDPV) referenence strains in GenBank,and phylogenetic tree analysis showed that all the strains of GPV shared highly common identity compared with each other,but there were also some difference between different strains.Meanwhile,GPV and MDPV probably share the common epitopes.

Key concepts: Epitope, Biology, GenBank, Goose, Phylogenetic tree, Gene, Sequence analysis, Molecular biology

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