Analysis of the Major Open Reading Frames' Nucleotide Sequence of Goose Parvovirus GPV-YG Strain Isolated in China

The two major open reading frames (ORF) of Goose parvovirus GPV-YG strain were amplified by overlapping PCR. The nucleotide sequence of PCR products was determined. The accession number of this sequence in GenBank is AF416726. The two major ORF's nucleotide sequence of GPV-YG has a 94% similarity to that of referential strain GPV-B, indicating they are closely related. And the characteristics of GPV-YG structural (VP) and non-structural (NS) proteins were analyzed. The VP proteins of GPV-YG and GPV-B share 96.4% similarity in amino acids sequences, and the NS proteins share 97.9% similarity. The two major ORFs of GPV-YG and MDPV-FM were also compared. They show 80% similarity in nucleotide sequences. The VP proteins of GPV-YG and MDPV-FM share 84.6% similarity in amino acids sequences, and the NS proteins share 89.6%.