Study on relationship between levels of IL-1β in follicular fluid and IVF-ET outcome in PCOS patients

Objective To investigate the effect of GnRH antagonist in IVF-ET on IL-1β in follicular fluid and the relationship between GnRH antagonist and IVF-ET outcome in polycystic ovarian syndrome(PCOS) patients.Methods Seventy-six cases of PCOS patients undergoing IVF-ET between August 2011 and September 2012 were divided randomly into two groups: Group A(study group) : 28 patients with PCOS were subjected to a protocol of gonadotropin-releasing hormone antagonist.Group B(control group) : 48 patients with PCOS were subjected to a long protocol of gonadotropin-releasing hormone agonist.The level of IL-β of the two groups and the relationships between GnRH antagonist and IVF-ET outcome were analyzed.Results The two groups were similar with respect to IL-1β in follicular fluid(P 0.05);the two groups were similar with respect to endometrium thickness,the number of oocyte,the mature rate of the oocytes,fertilization rates,cleavage rates,the rate of the high-quality embryos,implantation rate,pregnancy rate(P 0.05).The doses of gonadotropin and the days of administration in Group A was less than those in Group B(P 0.01).The levels of serum E2 on day of HCG administration,and the cycle cancellation rate in Group A was lower than that in Group B(P 0.05 or 0.01).The difference of the incidence rate of OHSS was not significant(P 0.05) between the two groups.Conclusion It is demonstrated that GnRH antagonist in PCOS patients undergoing IVF-ET did not alter levels of IL-1β in follicular fluid,the development of follicle and the oocyte competence.These results demonstrated the high-performance and safety of this drug on controlled ovarian stimulation protocol.