Expression of VEGF and CD34 in osteosarcoma tissue and its clinical significance

Objective:To explore the expression of VEGF and CD34 in osteosarcoma tissue and to analyze the association of VEGF with MVD,metastasis and prognosis of osteosarcoma.Methods:Immunohisto-chemical staining(SP method)was used to detect the expressions of VEGF and CD34 in specimens from 41 cases of osteosarcoma.To calculate microvessel density and overall analyze information of all the 41 cases.Results:In 41 cases,the positive rate of VEGF was 68.2%(28/41).The expression of VEGF was not significantly correlated to tumor's size,Price,s grade and Dahlin's difierentiation (P0.05);the expression of CD34 in all the 41 cases was positive. MVD (40.25±8.02)in VEGF positive patients was much higher than that(15.54±5.87)in VEGF negative patients(P0.05);the positive rate of VEGF (92.9%) in the patients with metastasis was remarkably higher than that (55.6%)in the patients without metastasis (P0.05);the five-year survival rate (7.1%)in the patients with positive expression of VEGFwas significantly lower than that (46.2%)in negative control groug (P0.05).Conclusion:High expression of VEGF is found in osteosarcoma tissue.The expression of VEGF is closely correlated to MVD,metastasis and prognosis of osteosarcoma.Detection of VEGF and MVD may be useful for judging metastasis and prognosis of osteosarcoma.