Expression of VEGF and BMP-2 in osteosarcoma tissue and its clinical significance

Objective:To explore the expression of VEGF and BMP-2 in osteosarcoma tissue and its association with diagnosis,metastasis/recurrence and prognosis of osteosarcoma.Methods:Immunohistochemical staining(SP method)was used to detect the expression of VEGF,BMP-2 and CD34 in specimens from 41 cases of osteosarcoma.Microvessel density was calculated,and overall information of all the 41 cases were analyzed.Results:In 41 cases,the positive rates of VEGF and BMP-2 were 68.2%(28/41)and 61.0%(25/41)respectively.The expression of VEGF and BMP-2 were not significantly correlated to tumor's size,Price's grade and Dahlin's difierentiation(P0.05).The positive rate of BMP-2 and MVD(75% and 40.25±8.02)in VEGF positive patients were respectively much higher than those(30.8%and 15.54±5.87)in VEGF negative patients(P0.05).The positive rates of VEGF and BMP-2(92.9%and 85.7%)in patients with metastasis / recurrence were respectively remarkably higher than those(55.6% and 48.1%)in patients without metastasis/recurrence(P0.05).The five-year survival rate in patients with positive expression of VEGF and BMP-2(7.1%and 4%)were significantly lower than that(46.2%and 43.8%)in negative control group(P0.05).Conclusion:High expression of VEGF and BMP-2 were found in osteosarcoma tissue.The expression of VEGF was closely correlated to the expression of BMP-2 and MVD.Detection of VEGF and BMP-2 expression and MVD may be useful for judging metastasis/recurrence and prognosis of osteosarcoma.