Comparison of VEGF,MVD and p53 Expression in Patients with Localized and Metastatic Osteosarcoma

Objective:To investigate VEGF,p53 expression and MVD in osteosarcoma and address the effect of VEGF and p53 mutation in the progression and metastases of osteosarcoma.Methods:The specimens from 57 patients with primary localized osteosarcoma and 27 patients with osteosarcoma that were metastatic at the time of diagnosis were stained immunohistochemically for VEGF and p53,MVD and counted,the results were comparatively analyzed.Results:The positive expression rates of VEGF and MVD in group of patients who presented with metastases were higher than in group of those who presented with a localized osteosarcoma( P =0.0494, P =0.0302);p53 expression did not differentiate in the two groups ( P 0.05);The tumors in the metastatic group tend to be larger than those in the local group ( P =0.0350);MVD was significantly higher in the VEGF or p53 positive tumors than in the negative ones( P =0.0125, P =0.0036);The conformable rate of VEGF and p53 expression was 51.2%; MVD was the highest in VEGF and p53 positive co expression tumors,and was lowest in those VEGF and p53 negative tumors,differences were significant( P =0.0077).Conclusion:Both VEGF and p53 mutation irritate angiogenesis in osteosarcoma.Osteosarcomas with more angiogenesis are more aggressive and earlier to metastasis.