Proliferation Inhibition of RBY79 Cells by Quercetin

Objective To investigate the effect of Quercetin on growth of the RB cell line Y79 In vitro and the corresponding mechanism involved in cell apoptosis.Methods Y79 was cultured in vitro and Inhibition rate of Quercetin on Y79 cells was assayed after 48 and 72 hours,then studied by MTT method to measure IC50 so as to observe the cytotoxicity of Quercetin.The morphology changes of the nucleons were observed,by Hoechst 33342/Pl staining.Results Proliferation of Y79 cells were significantly inhibited by Quercetin in the way of dose-effect relation.After the Y79 cells were treated by different concentrations of Quercetin for 48 and 72 hours,the IC50 was 148 μmol/L and 115 μmol/L by MTT experiments and distinct morphology changes of cell apoptosis such as karyopyknosis and conglomeration were observed by Hoechst 33342/Pl staining.Conclusion Quercetin inhibits proliferation of Y79 cells and induces cells specifically apoptosis.