Regulation of proliferation and protein expression in GTN treated tumor cells

Objective To investigate the proliferation and analyze the protein expression of the Hela cells by the regulation of glyceryl trinitrate (GTN) that acts as the donor of nitric oxide.Methods Cell morphology and MTT assay were used to analyze cell proliferation.Two-dimensional polyacrylamide gel electrophoresis (2-DE) and computer-assisted image analysis find the spots of interests. Difference of protein expression between GTN-treating group and control group was detected.Results Hela cells treated with 40μg/ml GTN in culture medium underwent inhibition of the growth activity, which were not clustered and shriveled. Some atypia cells were observed.Density of the cells was obviously decreased(0.0825, P0.05).The inhibition ratio of cell proliferation was 73.3% in MTT assay. The matching rate of the GTN group was 86%.Compared with control group, there were 312 protein spots changed greatly in GTN-treating group, which had 101(15.2%) protein spots up-regulated and 211(31.8%) protein spots down-regulated. 17 proteins were detected only in GTN-treating group cells and 21 proteins were detected only in control group cells.Conclusion Glyceryl trinitrate in certain concentration could inhibit the Hela cells in vitro and the spots of interests may be identified as the proteins that associate with regulation of GTN, which will be further analyzed.