Regulation of MMP-9/TIMP-1 Expression in High Concentration GTN Treated Tumor Cells

To investigate the regulation of MMP-9/TIMP-1 expressions in glyceryl trinitrate(GTN) treated Hela cells.The Hela cells were treated with GTN that acted as the donor of nitric oxide.The microarray technique was performed to analyze gene expression profiles related tumor angiogenesis and metastasis.MMP-9 and TIMP-1 gene expressions in treated Hela cells were examined through RT-PCR.ELISA assay was applied to determine the protein concentrations of MMP-9 and TIMP-1 in culture medium and calculate the MMP-9/TIMP-1 ratio.The results showed that some gene expressions were changed in Hela cells treated with 40μg/ml GTN in culture medium.The expression of MMP-9 in GTN group was greatly lower than that in control group,but the expression of TIMP-1 was not obviously changed.MMP-9 concentration in culture medium reduced dramatically,but no changed for TIMP-1.The ratio of MMP-9/TIMP-1 was altered statistically.The results indicate that glyceryl trinitrate in certain concentration could regulate the gene expression in tumor cell and shift the production balance of MMP-9 and TIMP-1.