Effects of Upregulated Expression of microRNA-205 on Biological Properties of HeLa Cells

【Objective】To investigate the effects of the upregulated expression of microRNA-205 (miR-205) on the biological properties of HeLa cells. 【Methods】There were four groups: miR-205 up-regulation group, negative control group, mock transfection group, and blank control group. Each kind of detection was repeated for three times. Through siPORT NeoFX transfection agent, the cells in miR-205 up-regulation group were transfected with Pre-miR-hsa-miR-205 miRNA precursor, while the cells in negative control group were transfected with Cy3 dye labeled PremiR negative control. The transfection efficiency was evaluated under fluorescence microscope. The miR-205 expression was measured by real time PCR. Cellular growth and proliferation capacity were assayed by the methyl thiazolyl tetrazolium (MTT) assay and colony formation assay, respectively. The apoptosis rate and cell cycle distribution were tested by flow cytometry. Cellular migration ability was detected by Transwell chamber assay. 【Results】 Real time PCR showed that the miR-205 expression in miR-205 up-regulation group increased 3061.5 folds after the transfection of hsa-miR-205 Precursor. Compared with that of blank control group, the numbers of live cells 72 h after transfection in miR-205 up-regulation group and negative control group were (126 ± 41)% and (126 ± 39)% respectively. In miR-205 up-regulation group and negative control group, the colony forming rates were (79 ± 11)% and (63 ± 10)% respectively, the apoptosis rates were (4.6 ± 2.1)% and (4.1 ± 2.4)% respectively, the cell proportions in S phase were (19.2 ± 3.6)% and (23.4 ± 3.0)% respectively, and the migration cells under 200 magnification field were (63 ± 17) and (68 ± 16) respectively. The cell proliferation ability was enhanced in miR-205 up-regulation group, while the cellular growth activity did not change compared with that in control group. The cell proportion in S phase decreased, while the apoptosis rate and the cellular migration ability did not show significant change. 【Conclusions】 After the up-regulated expression of miR-205, the cellular proliferation ability and the cell cycle change in HeLa cells, while the cellular growth, the apoptosis rate or the migration ability show no change.