Effects of Celecoxib on the proliferation,apoptosis and expression of Survivin in human lymphoma cell line Raji

【Objective】To investigate the effect of Celecoxib(a selective COX-2 inhibitor)on Raji cell line of human malignant lymphoma and the possible mechanism.【Methods】Human Burkitt's lymphoma cells Raji were cultivated in vitro and treated with various concentrations of Celecoxib.The growth of Raji cells was determined by MTT assay.Apoptosis rate,cell cycle distribution and expression of Survivin protein in Raji cells were detected by FCM.Expression of Survivin mRNA in Raji cells was detected by RT-PCR.【Results】The growth of Raji cells was inhibited by Celecoxib with concentration and time dependent manners from 12.5～150 μmol/L.Apoptosis peak was detected by FCM,and the apoptotic rate was between(9.20±0.19)% and(44.63±1.34)%.The cell percentage in G0/G1 phase increased,whereas the cell percentage in S and G2/M phase decreased,the apoptotic rate and cell cycle distribution were in concentration dependent manners.Celecoxib downregulated the expression of Survivin protein and Survivin mRNA in Raji cells.【Conclusions】The effect of Celecoxib inhibiting cell Raji and inducing cell apoptosis may be related to the blocking cell cycle progress and downregulating the expression of Survivin.