Effects of selective COX-2 inhibitor on proliferation,apoptosis and survivin expression in human lymphoma cell line Raji

Objectives To investigate the effect of celecoxib(a selective COX-2 inhibitor) on proliferation,apoptosis,cell cycle distribution and survivin expression in human malignant lymphoma cell line Raji and the possible antitumor mechanism in COX-2 independent pathway of celecoxib.Methods Human Burkitt's lymphoma cells Raji were cultivated in vitro and treated with various concentrations of celecoxib.Cell inhibitive rate was investigated by MTT assay.Apoptosis rate,cell cycle distribution and expression of survivin protein in Raji cells were detected by flow cytometry(FCM).Expression of survivin mRNA in Raji cells was detected by RT-PCR.Results The growth of Raji cells was inhib-ited by celecoxib in a concentration and time dependent manners within the range of 12.5 ～ 150 μmol / L.The typical apoptosis peak was detected by FCM,and the apoptotic rate was between(9.20 ± 0.19)% and(44.63 ± 1.34)%.The cell percentage in G0 / G1 phase increased,whereas the cell percentage in S and G2 / M phase decreased,the apoptotic rate and cell cycle distribution were in concentration dependent manners.celecoxib downregulated the expression of survivin protein and survivin mRNA in Raji cells.Conclusions The inhibitory effect of celecoxib on cell Raji and its ability to in-duce cell apoptosis may relate to its blocking of cell cycle progress and downregulating the expression of survivin,which may be a possible antitumor mechanism of celecoxib in COX-2 independent pathway.