Research on effect of selective COX-2 inhibitor celecoxib on proliferation,apoptosis in human lymphoma cell line raji

Objective:To investigate the possible antitumor mechanism of Celecoxib in COX-2 independent pathway,and to observe the effect of selective COX-2 inhibitor Celecoxib on proliferation,apoptosis,cell cycle distribution in human malignant lymphoma cell line Raji.Methods:Human Burkitt’s lymphoma cells Raji were cultivated in vitro and then treated with various concentrations of Celecoxib.Cell inhibitive rate was investigated by MTT assay.Apoptosis rate and cell cycle distribution were investigated by FCM.Results:Absorbance light degree in experiment groups after treated Raji cells for 24,48,72 h were significantly different compared with control group (P0.05).At the same time,there was a significant difference between concentration groups and time groups (P0.05).Apoptosis peak emerged significantly after Raji cells treated with Celecoxib for 48 hours.AP were significant higher in experiment groups than that in control group and AP were significantly different among concentration groups(P0.05).Compared with control group,cell cycle distribution in test groups of Celecoxib existed a significant difference(P0.05) and cell cycle distribution were significantly different among concentration groups(P0.05).Conclusion:The survival of Raji cells were inhibited by Celecoxib in vitro with a concentration and time dependent manners.Celecoxib induced cell cycle arrest in G1 phase and inhibited the growth of Raji cells,which may be one of the mechanisms of COX-2 independent pathway.