Severe acute pancreatitis complicated with intestinal injury in plateau hypoxia

Objective:To observe the intestinal mucosal tissue of rats with severe acute pancreatitis(SAP) in platuau hypoxia,and investigate the mechanism of gut barrier injury of rats with SAP at high altitude(H-SAP).Methods: Forty-eight Wistar rats were randomly divided into 2 groups(n=24 for each group),Xi'an group(X) and Maxianshan group(M) according to different altitude.Each group was further divided into four subgroups(n=6 for each subgroup),sham-operation group(SO group),SAP-6 h group,SAP-12 h group and SAP-24 h group,4% sodium taurocholate was injected into the pancreatic duct to induce SAP.Pancreas and intestine tissues were harvested to observe the pathological changes,and the content of TNF-α and IL-10 in intestine were measured respectively.The ultrastructure of intestine mucosa was observed under transmission electron microscope(TEM).Results:The expression of TNF-α and IL-10 in X-SAP group was significantly different with that in M-SAP group(P0.05).Under the light microscopy,injury of pancreas and intestine at different time point in M-SAP group was more obvious than that in X-SAP group(P0.05).The pathological scores of intestine were positively correlated with that of pancreas in SAP group(r=0.902,P0.01).Injury of intestinal mucosal was more obvious in M-SAP group than that in X-SAP group at each time point under TEM.Conclusion:Injury of intestinal mucosal barrier in rats with SAP can be induced and aggravated in plateau hypoxia.Disturbance of TNF-α and IL-10 expression play an important role in the pathogenesis and development of intestinal mucosal barrier injury in rats with H-SAP.