Isolation,Purification and Monosaccharide Composition Analysis of Polysaccharide from Fruiting Bodies of Phellinus Igniarius (L.ex.Fr.) Quel

Objective: To study the monosaccharide composition of the bioactive polysaccharide extracted from the fruiting body of Phellinus igniarius. Methods: The process of isolating a water soluble crude polysaccharide was achieved by hot water extraction, filtration, ethanol precipitation and freeze-drying. The crude polysaccharide was purified to obtain PIFI by chroma- tography on DEAE-Sepharose Fast Flow column and Sephacryl S-100 High Resolution column. The monosaccharide compo- sition and molecular ratio of the PIFI were analyzed by capillary gas chromatography and mass spectrum. Results: The monosaccharide compositions of PIFI are 3(4)-O-methyl-hexasaccharide, mannose, glucose, and galactose with the molar ratio of 0.93:0.64:1:1.94. Conclusion: The determination method of monosaccharide composition of the PIFI by capillary gas chromatography and mass spectrum is established, and the results of monosaccharide composition and molar rate of the PIFI are obtained. 3(or 4)-O- methyl-hexasaccharide is present in the PIFI.