2014•GuihaiaRequires access

Analysis of monosaccharide compositions of Lentinus edodes polysaccharide by HPLC

Shan Wu

Abstract

Monosaccharide composition in Lentinus edode polysaccharides was determined by high performance liquid chromatography(HPLC).The L.edodes polysaccharide was extracted using ultrasonic-assisted extraction.Monosaccharide were derived by 1-phenyl-3-methyl-5-pyrazolone(PMP)and the PMP derivatives of monosaccharide were analyzed by HPLC.The results showed that L.edodes polysaccharide was composed of mannose,ribose,rhamnose,glucose,galactose and xylose and their molar ratio was about 1.00∶0.90∶0.91∶28.03∶1.58∶0.11.The method was rapid,simple and reproducible and could be used to determine monosaccharide compositions of L.edodes.

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Key concepts: Lentinus, Monosaccharide, Polysaccharide, Rhamnose, Chemistry, High-performance liquid chromatography, Chromatography, Xylose