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2004•The Research of Environmental SciencesRequires access

Study on the Method of Human Buccal Cell Comet Assay

Lu Zhisong, Yan Qiao, Yan Yan, Kang Yanyong, Yang Xu

Open publisher page 2 citations

Abstract

In order to establish a sensitive and rapid assay to test the genotoxicity of environmental genotoxin on human target cells,the authors carried out a study on the optimal duration and temperature of exposure treatment and two testing protocols for crosslinks.Results show that after exposed to 7.5 μmol/L of formaldehyde at 37 ℃ for 30 and 60 min,DNA was damaged more acutely than that for 15 min.After exposet to 7.5 μmol/L of formaldehyde at 4,23 and 37 ℃ for 30 min,DNA damage happened in all of the three groups,but at 37 ℃ DNA strands broke most.On the two testing protocols for crosslinks,increasing the duration of electrophoresis is more reasonable for the detecting of DNA breakage and crosslinks.

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What this paper is about

In order to establish a sensitive and rapid assay to test the genotoxicity of environmental genotoxin on human target cells,the authors carried out a study on the optimal duration and temperature of exposure treatment and two testing protocols for crosslinks.Results show that after exposed to 7.5 μmol/L of formaldehyde at 37 ℃ for 30 and 60 min,DNA was damaged more acutely than that for 15 min.After exposet to 7.5 μmol/L of formaldehyde at 4,23 and 37 ℃ for 30 min,DNA damage happened in all of the three groups,but at 37 ℃ DNA strands broke most.On the two testing protocols for crosslinks,increasing the duration of electrophoresis is more reasonable for the detecting of DNA breakage and crosslinks.

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Available abstract

In order to establish a sensitive and rapid assay to test the genotoxicity of environmental genotoxin on human target cells,the authors carried out a study on the optimal duration and temperature of exposure treatment and two testing protocols for crosslinks.Results show that after exposed to 7.5 μmol/L of formaldehyde at 37 ℃ for 30 and 60 min,DNA was damaged more acutely than that for 15 min.After exposet to 7.5 μmol/L of formaldehyde at 4,23 and 37 ℃ for 30 min,DNA damage happened in all of the three groups,but at 37 ℃ DNA strands broke most.On the two testing protocols for crosslinks,increasing the duration of electrophoresis is more reasonable for the detecting of DNA breakage and crosslinks.

Key concepts: Genotoxicity, Comet assay, Buccal swab, DNA damage, Formaldehyde, DNA, Buccal administration, Chemistry

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