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2001•Carcinogenesis,Teratogenesis and MutagenesisRequires access

STUDY ON THE EFFECTS OF DNA CROSSLINKS OF FORMALDEHYDE AND MITOMYCINE C

Zun Zhang

Open publisher page 1 citations

Abstract

Purpose: To explore the time course effects in detecting DNA crosslinks with comet assay and to prove the feasibility of comet assay for detecting DNA crosslinks. Method: Hydrogen peroxide (H\-2O\-2) was used as standard DNA breaking agent, the relationship of time effects of DNA crosslinks in TK6 cells induced by formaldehyde (FA) was studied using comet assay. Meanwile, two kinds of DNA crosslinking agents (FA and mitomycine c) were tested and verified. Results: The length of DNA migration caused by H\-2O\-2 decreased with the increase in the dosage of FA and MMC within the doses given. The investigation of time course effects of DNA crosslinks showed that the action of DNA crosslinks increased with increase in the exposure time of FA. Conclusion: The comet assay could detect both DNA protein crosslinks and DNA DNA crosslinks. The comet assay was more sensitive for detecting DNA protein crosslinks than detecting DNA DNA crosslinks.

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What this paper is about

Purpose: To explore the time course effects in detecting DNA crosslinks with comet assay and to prove the feasibility of comet assay for detecting DNA crosslinks. Method: Hydrogen peroxide (H\-2O\-2) was used as standard DNA breaking agent, the relationship of time effects of DNA crosslinks in TK6 cells induced by formaldehyde (FA) was studied using comet assay. Meanwile, two kinds of DNA crosslinking agents (FA and mitomycine c) were tested and verified. Results: The length of DNA migration caused by H\-2O\-2 decreased with the increase in the dosage of FA and MMC within the doses given. The investigation of time course effects of DNA crosslinks showed that the action of DNA crosslinks increased with increase in the exposure time of FA. Conclusion: The comet assay could detect both DNA protein crosslinks and DNA DNA crosslinks. The comet assay was more sensitive for detecting DNA protein crosslinks than detecting DNA DNA crosslinks.

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Available abstract

Purpose: To explore the time course effects in detecting DNA crosslinks with comet assay and to prove the feasibility of comet assay for detecting DNA crosslinks. Method: Hydrogen peroxide (H\-2O\-2) was used as standard DNA breaking agent, the relationship of time effects of DNA crosslinks in TK6 cells induced by formaldehyde (FA) was studied using comet assay. Meanwile, two kinds of DNA crosslinking agents (FA and mitomycine c) were tested and verified. Results: The length of DNA migration caused by H\-2O\-2 decreased with the increase in the dosage of FA and MMC within the doses given. The investigation of time course effects of DNA crosslinks showed that the action of DNA crosslinks increased with increase in the exposure time of FA. Conclusion: The comet assay could detect both DNA protein crosslinks and DNA DNA crosslinks. The comet assay was more sensitive for detecting DNA protein crosslinks than detecting DNA DNA crosslinks.

Key concepts: Comet assay, DNA, Formaldehyde, DNA damage, Chemistry, Molecular biology, Biochemistry, Hydrogen peroxide

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STUDY ON THE EFFECTS OF DNA CROSSLINKS OF FORMALDEHYDE AND MITOMYCINE C — Research Paper | ScholarLens