RP-HPLC Determination of 3 Flavonoid Aglycones of Zanthoxylum Bungeanum

RP-HPLC was applied to the determination of 3 flavonoid aglycones,including quercetin, kaempferol and isorhamnetin in zanthoxylum bungeanum.The sample solution was separated by using Zorbax Eclipse C_(18) column(4.6 mm×150 mm,5μm) as stationary phase and a mixture of methanol and 0.4%(φ) phosphoric acid solution(mixed 1 to 1 by vol.) as mobile phase.The contents of the 3 flavonoid aglycones were determined at the wavelength 360 nm.Linear relationships between values of peak area and mass concentration of quercetin,kaempferol and isorhamnetin were kept in ranges of 2.19-109.6 mg·L~(-1),2.28-114.0 mg·L~(-1) and 2.80-140.0 mg·L~(-1),respectively.Tests for recovery and precision were made by standard addition method. Values of recovery and RSD's(n = 5) found were 95.6%and 2.98%for quercetin,104.4%and 3.97%for kaempferol,103.8%and 4.30%for isorhamnetin,respectively.