RP-HPLC Determination of 3 Flavonoid Aglycone Hydrolyzates of Leaves of Microcos Paniculata L.from Different Origins

RP-HPLC was applied to the simultaneous determination of 3 flavonoid aglycone hydrolyzates including quercetin,kaempferol and isorhamnetin by hydrolysis of leaves of Microcos paniculata L.from different regions.The hydrolyzed solution of sample was separated by gradient elution with methanol and 0.4%(φ) phosphoric acid solution on Kromasil C18 column(250 mm×4.6 mm,5 μm),and their contents determined at the wavelength 368 nm.Linear relationships between values of peak area and mass concentration of quercetin,kaempferol and isorhamnetin were kept in ranges of 1.97-19.7 mg·L-1,2.02-20.2 mg·L-1,and 2.02-20.2 mg·L-1,respectively.Tests for recovery and precision were made by standard addition method,giving values of recovery in the range of 93.0%-99.6%,(quercetin),92.6%-99.8%(kaempferol) and 92.5%-99.5%(isorhamnetin).Values of RSD′s(n=6) found were 2.48% for quercetin,2.80% for kaempferol and 3.17% for isorhamnetin.