Extraction technology of polysaccharides from phellinus linteus by ultrasonic-assisted complex enzymatic method

Ultrasonic-assisted complex enzymatic method was used to optimize the extraction conditions of polysaccharides from phellinus linteus.Using the extraction yield of total polysaccharides as target,the process was optimized by investigating the influence of ultrasonic time,complex enzyme dosage,enzymolysis time,enzymolysis temperature and pH value.Results: Results showed that the optimal extraction conditions for polysaccharides from phellinus linteus by ultrasonic-assisted complex enzymatic method were: ultrasonic time 300 s,2.0% caroid,2.0% pectinase plus 2.0% cellulase,extraction temperature 50 ℃,extraction time 90 min and pH 4.0,and in this condition,the polysaccharide yield could reach 1.46%.