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2011•China Medical HeraldRequires access

Content determination of Paeoniflorin in Huoxue Tongmai Tablets by HPLC

Xiong Xin

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Abstract

Objective: To establish a method for the content determination of Paeoniflorin in Huoxue Tongmai Tablets by HPLC.Methods: The radix Paeoniae rubra was quantitative analysed by HPLC.The C18(4.6 mm×250 mm,5 μm) column was used.The mobile phases consisted of methanol and water(27∶73),the flow rate was 0.8 ml/min and the detection wavelength was at 230 nm.The temperature was 30℃.Results: The optimum linear relation of Peoniflorin was 0.98-7.35 μg,the average recovery rate was 97.52% with a RSD of 1.57%.Conclusion: The method is a accurate,recurring and easy to use assay for quality control.

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What this paper is about

Objective: To establish a method for the content determination of Paeoniflorin in Huoxue Tongmai Tablets by HPLC.Methods: The radix Paeoniae rubra was quantitative analysed by HPLC.The C18(4.6 mm×250 mm,5 μm) column was used.The mobile phases consisted of methanol and water(27∶73),the flow rate was 0.8 ml/min and the detection wavelength was at 230 nm.The temperature was 30℃.Results: The optimum linear relation of Peoniflorin was 0.98-7.35 μg,the average recovery rate was 97.52% with a RSD of 1.57%.Conclusion: The method is a accurate,recurring and easy to use assay for quality control.

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Available abstract

Objective: To establish a method for the content determination of Paeoniflorin in Huoxue Tongmai Tablets by HPLC.Methods: The radix Paeoniae rubra was quantitative analysed by HPLC.The C18(4.6 mm×250 mm,5 μm) column was used.The mobile phases consisted of methanol and water(27∶73),the flow rate was 0.8 ml/min and the detection wavelength was at 230 nm.The temperature was 30℃.Results: The optimum linear relation of Peoniflorin was 0.98-7.35 μg,the average recovery rate was 97.52% with a RSD of 1.57%.Conclusion: The method is a accurate,recurring and easy to use assay for quality control.

Key concepts: Paeoniflorin, Medicine, Chromatography, High-performance liquid chromatography, Content determination, Radix (gastropod), Linear relationship, Traditional medicine

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