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2008•Medical Journal of the Chinese People's Armed Police ForcesRequires access

Determination of paeoniflorin in Tiaojing Huoxue Tablets by HPLC

Zhao Li-hua

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Abstract

Objective To establish an HPLC method for determination of paeoniflorin in Tiaojing Huoxue Tablets.Methods A Waters C18 column (250 mm×4.6 mm, i.d.5 μm) was used,with mobile phase of acetonitrile 0.05 mol and L KH2PO4(15∶85),a flow rate of 1.0 ml/min,detection wavelength at 230 nm, and column temperature at 25℃.Results Paeoniflorin concentration in the range of 0.01～0.50 mg/ml was linear with peak area (r=0.9 999). The mean recovery was 98.84%,RSD=1.37% (n=6).Conclusions The method is sensitive,accurate,and reproducible;it can be used as the quality control index for Tiaojing Huoxue Tablets.

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Objective To establish an HPLC method for determination of paeoniflorin in Tiaojing Huoxue Tablets.Methods A Waters C18 column (250 mm×4.6 mm, i.d.5 μm) was used,with mobile phase of acetonitrile 0.05 mol and L KH2PO4(15∶85),a flow rate of 1.0 ml/min,detection wavelength at 230 nm, and column temperature at 25℃.Results Paeoniflorin concentration in the range of 0.01～0.50 mg/ml was linear with peak area (r=0.9 999). The mean recovery was 98.84%,RSD=1.37% (n=6).Conclusions The method is sensitive,accurate,and reproducible;it can be used as the quality control index for Tiaojing Huoxue Tablets.

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Available abstract

Objective To establish an HPLC method for determination of paeoniflorin in Tiaojing Huoxue Tablets.Methods A Waters C18 column (250 mm×4.6 mm, i.d.5 μm) was used,with mobile phase of acetonitrile 0.05 mol and L KH2PO4(15∶85),a flow rate of 1.0 ml/min,detection wavelength at 230 nm, and column temperature at 25℃.Results Paeoniflorin concentration in the range of 0.01～0.50 mg/ml was linear with peak area (r=0.9 999). The mean recovery was 98.84%,RSD=1.37% (n=6).Conclusions The method is sensitive,accurate,and reproducible;it can be used as the quality control index for Tiaojing Huoxue Tablets.

Key concepts: Paeoniflorin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetonitrile, Detection limit

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