Effect of oxysophocarpineon proliferation and apoptosis of human breast cancer cell

Objective To investigate the effect of oxysophocarpine( OSC) on proliferation and apoptosis of human breast cancer cell( michigan cancer foundation- 7,MCF- 7). Method There were divided into groups of final oxysophocarpine concentration of 10,20,50,100,200,400,500 μmol ·L~(-1)and the control group. MTT method was used to analyze the inhibitory effect of on the inhibition of MCF- 7 in different concentrations in the experimental groups and control group.Cell apoptosis and cell cycle were detected by flow cytometry. The effect of Caspase- 3 concentration was detected by Western blot. Results The proliferation inhibition rate of OSC on MCF- 7 cell reached 81. 80%,and apoptosis rate 81. 67%,showing concentration dependent. Half inhibitory concentration for MCF- 7 was 165. 31 μmol ·L~(-1). OSC had significant effects on the apoptosis of MCF- 7,which could make the number of MCF- 7 in G0- G1 gradually increase,and the number of G2- M phase and S phase decrease gradually. OSC could enhance the expression of Caspases- 3 protein in MCF- 7 cells dose- dependently. Conclusion OSC may inhibit the proliferation of cells by up- regulating the expression of Caspases- 3 and changing the cell cycle of MCF- 7,thus inhibiting the proliferation of cells and promoting the apoptosis of MCF- 7 cells.