Study on Reducing Cost of Cotoneaster hjelmqvistii Plantlet in vitro

Taking Cotoneaster hjelmqvistii as material,the soft white sugar and tap water were respectively used to instead of sucrose and distilled water in micro-shoot proliferation culture to reduce the cost of Cotoneaster hjelmqvistii plantlet in vitro.The results showed that the micro-shoots multiplication had no significant difference among the treatments;during rooting culture with soft white sugar instead of sucrose,there was no significant difference among all treatments.Cotoneaster plantlets were placed in different culture environments for rooting,and under greenhouse the rooting effect was better than that under the training room environment.In conclusion,the soft white sugar and the tap water can be used to replace sucrose and distilled water for the Cotoneaster hjelmqvistii plantlets proliferation culture,also the soft white sugar can be used to replace sugar in the plantlet rooting.The plantlet rooting can be carried out under the greenhouse conditions.These methods can significantly reduce the cost of Cotoneaster hjelmqvistii plantlet in vitro.