Study on the effect of white granulated sugar on the plantlets rooting of Hemerocallis hybrida ‘Baltimore oriole’under tissue culture

Instead of sucrose using white granulated sugar,the action of white granulated sugar on the plantlets rooting of Hemerocallis hybrida‘Baltimore oriole’was studied.The plantlets were divided into two types: the caespitose shoot and the single shoot,and were respectively inoculated in rooting media 1/2MS+0 which contain different sugar concentration.The result showed that the action of white granulated sugar on the rooting rate and the number of the rooting were obvious.The plantlets in no sugar media became yellow and weak,and with the worst growth potential,the rooting rate no matter caespitose shoot or single shoot was zero.2.5% concentration of sugar was suitable for single shoot rooting,the rooting rate reached at 95%,the average count of the rooting was 3.59 in each plant.3.5% concentration of sugar was suitable for caespitose shoot rooting,the rooting rate reached at 90%,the average count of the rooting was 7.50 in each plant.The sugar concentration for caespitose shoot rooting was higher than single shoot rooting.The caespitose shoot had the largest rooting speed at the culture period from 14 days to 21 days,and for single shoot,the largest rooting speed period was from 7 days to 14 days.