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Gene expression and antibody preparation of chicken interferon (IFN)- γ.

Yan Zeng, Xiaofeng Ren, Cai HaoFan, Jiu Ze, Li GuangXing

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Abstract

The cDNA of chicken IFN-γ gene was cloned into pET30a vector to construct recombinant plasmid pET-30a-CHIFN-γ.The recombinant plasmid was transformed into E.coli BL21(DE3)and the bacterium was induced with IPTG.It was demonstrated by SDS-PAGE that the soluble protein was expressed in E.coli BL21(DE3).This protein was purified by Ni-NTA and used to prepare the antibody against chicken IFN-γ protein.The Western blotting result showed that the antibody could react with purified recombinant chicken IFN-γ protein.

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What this paper is about

The cDNA of chicken IFN-γ gene was cloned into pET30a vector to construct recombinant plasmid pET-30a-CHIFN-γ.The recombinant plasmid was transformed into E.coli BL21(DE3)and the bacterium was induced with IPTG.It was demonstrated by SDS-PAGE that the soluble protein was expressed in E.coli BL21(DE3).This protein was purified by Ni-NTA and used to prepare the antibody against chicken IFN-γ protein.The Western blotting result showed that the antibody could react with purified recombinant chicken IFN-γ protein.

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Available abstract

The cDNA of chicken IFN-γ gene was cloned into pET30a vector to construct recombinant plasmid pET-30a-CHIFN-γ.The recombinant plasmid was transformed into E.coli BL21(DE3)and the bacterium was induced with IPTG.It was demonstrated by SDS-PAGE that the soluble protein was expressed in E.coli BL21(DE3).This protein was purified by Ni-NTA and used to prepare the antibody against chicken IFN-γ protein.The Western blotting result showed that the antibody could react with purified recombinant chicken IFN-γ protein.

Key concepts: Recombinant DNA, lac operon, Molecular biology, Complementary DNA, Plasmid, Antibody, Biology, Gene

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