Effects of nimesulide on the proliferation and cell cycle distribution of human gastric cancer cell lines SGC7901

Objective To evaluate the potential role of nimesulide on the proliferation and cell cycle distribution of gastric adeno-carcinoma cell lines.Methods The human gastric cancer cell lines SGC7901 were employed in the study,PGE2 in the culture supernatant was measured by radioimmunoassay,After treatment with nimesulide,the inhibitory effect of nimesulide on the proliferation of cancer cells was quantified by MTT assay,and the cell cycle distribution of tumor cells was detected by flow cytometry.Results Nimesulide decreased the release of PGE2,Nimesulide inhibited the proliferation of the tumor cells in time and dose-dependent manners,Nimesulide changed cell cycle distribution of cells,the proliferation index of cells was obviously decreased also.Conclusion Nimesulide may inhibit the division and proliferation of cancer cells through the inhibition of Cyclooxygenase-2(COX-2) activity and cell circle growth,resulting in the apoptosis of tumor cells and interfering in metastasis and may play an important role in the of SGC7901 cells of gastric cancer.