Study the Effect of Nimesulide on Cell Proliferation of SGC-7901 Cell Line and Expression of hTERT-mRNA

Objective To study the effects of the selective COX-2 inhibitor(Nimesulide)on cell proliferation and expression of human telomerase reverse transcriptase messenger ribonucleic acid in the human gastric cancer cell line SGC-7901 and to explore the molecular mechanism of the antitumor activity of the selective COX-2 inhibitor.Methods After SGC-7901 was treated with different concentrations of Nimesulide(0、50、100、200、400 μmol/L) for different cultural time in vitro,the cellurlar proliferation was evaluated by MTT assay and the hTERT-mRNA expression was detected by RT-PCR.The cell morphology and growth manner was observed under phase contrant microscope.Results Nimesulide could inhibit the growth of SGC-7901 gastric cancer cell line in the time and dose-dependent manner;and the expression of hTERT-mRNA were decreased in dose-dependent manner;Compared with control group,it decreased significantly(P0.05).Conclusion Nimesulide decreased the expression of hTERT-mRNA in the gastric cancer SGC-7901 cell line,these indicated Nimesulide is a probably telomerase inhibitor.