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2001•Journal of Comprehensive StomatologyRequires access

The effects of mouse recombinant Periostin on proliferation and ALP activity of PDL cells.

Wu Zhi

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Abstract

Objective:To investigate the effects of mouse recombinant Periostin on proliferation and ALP activity of periodontal ligament cells (PDL cells).Methods:MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells.Results:Significant proliferation of PDL cells could be observed on Periostin at dose 40ug/ml ( P 0.01) and the ALP activity was increased at dose10μg/ml～80μg/ml( P 0.05).Conclusion:Periostin can promote the proliferation and ALP activity of PDL cells,but the effects need further studies. [

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Objective:To investigate the effects of mouse recombinant Periostin on proliferation and ALP activity of periodontal ligament cells (PDL cells).Methods:MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells.Results:Significant proliferation of PDL cells could be observed on Periostin at dose 40ug/ml ( P 0.01) and the ALP activity was increased at dose10μg/ml～80μg/ml( P 0.05).Conclusion:Periostin can promote the proliferation and ALP activity of PDL cells,but the effects need further studies. [

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Available abstract

Objective:To investigate the effects of mouse recombinant Periostin on proliferation and ALP activity of periodontal ligament cells (PDL cells).Methods:MTT method and enzyme dynamics method were used to observe the ability of proliferation and ALP expression of PDL cells.Results:Significant proliferation of PDL cells could be observed on Periostin at dose 40ug/ml ( P 0.01) and the ALP activity was increased at dose10μg/ml～80μg/ml( P 0.05).Conclusion:Periostin can promote the proliferation and ALP activity of PDL cells,but the effects need further studies. [

Key concepts: Periostin, Periodontal fiber, Recombinant DNA, Chemistry, Cell growth, MTT assay, Molecular biology, Cell biology

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