Effects of IL-1 β on the Biological Activity of Human Periodontal Ligament Cells in Vitro

Objective To explore the effects of interleukin- 1β(IL-1β)on the biological activity of human periodontal ligament(PDL) cells in vitro. Methods Human PDL cells were cultured in DMEM medium containing IL-1β(0.1,0.5,1,5 and 10ng/ml) for 1,2,3,4 and 5 days, respectively. The proliferation of PDL cells was measured by MTT assay at the first, second, third, fourth and fifth days after IL-1β treatment, respectively. Fibronectin level in the medium was determined by ELISA at the fourth days after 10ng/ml IL-1β treatment, and alkaline phosphatase(ALP) activity was measured by enzyme kinetic method. Results IL-1β inhibited the growth of human PDL cells in a dose-dependent manner, and its lowest effective concentration was 1ng/ml(P0.05). IL-1β singnificantly inhibited the fibronectin synthesis and ALP activity in human PDL cells(P0.001). Conclusion IL-1β may play an important role in the pathogenesis and repair of periodontal diseases.