Observation on the Hemostasis Effect of Collagen/fibrin Composite Hemostasis Paste

Objective To study the hemostasis effect of collagen and fibrin combination in New Zealand rabbit,and to compare its effect with collagen sponge.Methods Hemostasis experiments on ear arterial and venous bleeding,wound surface of the ear,incision of the femoral artery,injury of liver and wound surface of body were carried out on New Zealand rabbits.At the same time,its effect of hemostasis was compared with collagen sponge in terms of bleeding time,blood loss and binding of wound and dressings.Also,the healing of wound,its absorption in the body and anti-inflammatory effect were regularly observed.Results Compared to control group,both collagen /fibrin composite hemostatic(bandage) and collagen sponge had significant differences in hemostasis time of the ear artery and the ear vein,the wound surface of ear,the incision of the femoral artery and the injury of liver(P 0.05).Compared with the collagen sponge group,the composite hemostatic paste group had a significant difference in the hemostasis time of either ear arterial and venous bleeding or the wound surface of ears in New Zealand rabbit(P 0.05).Compared to the control group,the blood loss of the wound surface of ear and the injury of liver,group of collagen and fibrin composite had a significantdifference.It was observed that both collagen /fibrin composite hemostatic collagen paste and collagen sponge could be completely absorbed within 21 days without inflammatory response under the condition of either standard trauma of liver in vivo or superficial trauma.Conclusion The collagen and fibrin combination hemostatic had a significant hemostasis effect in the ear arterial and venous bleeding,the wound surface of ears,the injury of femoral artery and the injury of liver in New Zealand rabbit.And the superficial wound recovered well.The paste was absorbed fast in the body and had a certain anti-inflammatory effect.Furthermore,its hemostatic effect was better than collagen sponge in ear artery and ear vein cut and superficial wound of the ear in New Zealand rabbit.Thus,the collagen and fibrin composite hemostatic paste may be a kind of safe and effective local haemostasis biological material.