Optimization of Extraction Technology for Effective Parts from Sophora tonkinensis by Orthogonal Test
Weiyu Zhang
Abstract
Weiyu Zhang
Abstract
Objective:To optimize extraction technology of effective parts from Sophora tonkinensis.Method:Taking the content of total alkaloids as index,with extraction time,extraction times,ethanol concentration and amount as factors,extraction technology of total alkaloids from S.tonkinensis was optimized by L9(34)orthogonal test;With the content of total polysaccharides as index,taking the amount of water,extraction time and times as factors,extraction technology of total polysaccharides was optimized by orthogonal test.Result:Optimal extraction technology of total alkaloids was as following:extracted 2 times with 6 times the amount of 70% ethanol,1 h per time;Optimum extraction technology of total polysaccharides was as following:extracted 3 times with 6 times the amount of water,1.0 h each time.Conclusion:This optimized extraction technology was stable and feasible,it was suitable for industrial production with a good prospect.
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Objective:To optimize extraction technology of effective parts from Sophora tonkinensis.Method:Taking the content of total alkaloids as index,with extraction time,extraction times,ethanol concentration and amount as factors,extraction technology of total alkaloids from S.tonkinensis was optimized by L9(34)orthogonal test;With the content of total polysaccharides as index,taking the amount of water,extraction time and times as factors,extraction technology of total polysaccharides was optimized by orthogonal test.Result:Optimal extraction technology of total alkaloids was as following:extracted 2 times with 6 times the amount of 70% ethanol,1 h per time;Optimum extraction technology of total polysaccharides was as following:extracted 3 times with 6 times the amount of water,1.0 h each time.Conclusion:This optimized extraction technology was stable and feasible,it was suitable for industrial production with a good prospect.
Key concepts: Extraction (chemistry), Polysaccharide, Chromatography, Sophora, Water extraction, Chemistry, Mathematics, Biochemistry