2013•Hubei nongye kexueRequires access

Optimization of the Microwave-assisted Extraction Technology of Polysaccharides from Sophora tonkinensis

Qingsong Li

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Abstract

Orthogonal experimental design was used to optimize the extraction technology of polysaccharides from Sophora tonkinensis Gapnep..The content of total polysaccharides was determined by phenol-vitriolic colorimetry.The optimum extraction technology of polysaccharide was as followed,solid to liquid ratio,1∶10(m/V,g∶mL);extraction time,90 min;extracting twice.The content of polysaccharide was 3.62%.The method was simple,accurate,sensitive and suitable for extraction polysaccharide from S.tonkinensis.

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Orthogonal experimental design was used to optimize the extraction technology of polysaccharides from Sophora tonkinensis Gapnep..The content of total polysaccharides was determined by phenol-vitriolic colorimetry.The optimum extraction technology of polysaccharide was as followed,solid to liquid ratio,1∶10(m/V,g∶mL);extraction time,90 min;extracting twice.The content of polysaccharide was 3.62%.The method was simple,accurate,sensitive and suitable for extraction polysaccharide from S.tonkinensis.

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Available abstract

Orthogonal experimental design was used to optimize the extraction technology of polysaccharides from Sophora tonkinensis Gapnep..The content of total polysaccharides was determined by phenol-vitriolic colorimetry.The optimum extraction technology of polysaccharide was as followed,solid to liquid ratio,1∶10(m/V,g∶mL);extraction time,90 min;extracting twice.The content of polysaccharide was 3.62%.The method was simple,accurate,sensitive and suitable for extraction polysaccharide from S.tonkinensis.

Key concepts: Polysaccharide, Extraction (chemistry), Sophora, Chromatography, Colorimetry, Chemistry, Biochemistry, Medicine

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